The enzyme (2-5 A synthetase) which synthesizes ppp(A2′p)n A where n = 2 to 4 (collectively referred to as 2-5A)1 is widely distributed in a variety of cells and tissues in amounts which increase in response to interferon2–5 and vary with growth and hormone status6. 2-5A activates a nuclease which inhibits protein synthesis7–10. The non-phosphorylated ‘core’ of 2-5A ((A2′p)nA， were not found in significant amounts. The natural occurrence of core has not been described previously. 。

which can also be detected using the RB assay， n = 2 to 4) can inhibit DNA synthesis and cell growth11，12. Here we describe convenient and sensitive radioimmune (RI) and radiobinding (RB) assays for core and 2-5A. In combination with more satisfactory high performance liquid chromatography (HPLC) methods using reverse-phase C18 columns，。

these assays have been used to detect core and 2-5A in crude extracts from interferon-treated cells. The novel 2-5A synthetase products NAD2′p5′A2′p5′A and A5′p45′A2′p5′A2′p5′A (ref. 13)。

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